Page:NIOSH Manual of Analytical Methods - 3600.pdf/1
MANEB Dermal Patch C4 H6 N2 S4 Mn
MW: 265.2
METHOD: 3600, Issue 1
3600
CAS: 12427-38-2
RTECS: OP07000
EVALUATION: PARTIAL
OSHA : not applicable NIOSH: not applicable ACGIH: not applicable
PROPERTIES:
Issue 1: 15 March 2003
powder, yellow, wettable; sparingly soluble in water and organic solvents; vp not significant
NAMES & SYNONYMS: manganous ethylenebis(dithiocarbamic acid), Manzate; Dithane M-22
SAMPLING
MEASUREMENT
PATCH Dry NuGauze®, spun polyester gauze patch (see Figure 1)
TECHNIQUE:
HPLC, UV detection
ANALYTE:
ethylenebisdithiocarbamate anion
FLOW RATE:
not applicable
EXTRACTION:
VOL-MIN: -MAX:
not applicable not applicable
40 mL desorbing solution (1% Lcysteine, 3% Na 4 EDTA C2H2 O in water) in 50 to 65-mL wide-mouth bottles with Teflon®-lined screw caps
SHIPMENT:
desorb immediately in shipping bottles, pack in blue ice, cool to 4 °C, ship via overnight express
SAMPLER:
SAMPLE STABILITY:
at least 1 week at 4 °C
BLANKS:
2 to 10 field blanks per set ACCURACY
RANGE STUDIED:
not applicable
BIAS:
not determined
INJECTION VOLUME:
100 µL
MOBILE PHASE:
0.0675 M phosphate, 0.0525 M NaClO4 , pH 6.9, 1 g/L Na2 EDTA C2H2 O, 2 mL/min
COLUMN:
Dionex AS7 anion exchange column or equivalent, GS7 guard column
DETECTOR:
UV, 285 nm (8 max) or 254 nm
CALIBRATION:
solutions of Maneb in 1% L-cysteine, 3% Na2 EDTA
RANGE:
0.02 to 4 mg/sample [1]
OVERALL PRECISION ( Ö r T ):
not determined
ESTIMATED LOD:
0.02 mg/sample (0.5 µg/mL) [1]
ACCURACY:
not determined
PRECISION ( þ r ):
0.015 [1]
APPLICABILITY: This method determines Maneb collected on dry Nu Gauze® (spun polyester gauze) dermal patches attached to clothing on the arm(s), leg(s), or torso of agricultural workers. It is applicable to the determination of Zineb, Mancozeb, and Nabam since these all convert to the same analyte when dissolved in EDTA solution. INTERFERENCES: Not thoroughly investigated. No interferences have been found during method development.
OTHER METHODS: Maneb may also be determined by first methylating followed by HPLC analysis with a C18 column [2-5].
NIOSH Manual of Analytical Methods, Fourth Edition