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RIBAVIRIN

C8H12 N4O5

MW: 244.21

METHOD: 5027, Issue 2

CAS: 36791-04-5 EVALUATION:

OSHA : no PEL NIOSH: no REL ACGIH: no TLV

5027

RTECS: XZ4250000

UNRATED

PROPERTIES:

Issue 1: 15 May 1989 Issue 2: 15 August 1994

solid; MP 170 °C; VP negligible; sol. (water): 142 mg/mL @ 25 °C

SYNONYMS: 1-ß-D-ribofuranosyl-1,2,4-triazole-3-carboxamide; Virazole; ICN 1229

SAMPLING SAMPLER:

FILTER (1-µm, 37-mm glass fiber)

MEASUREMENT TECHNIQUE:

HPLC, UV DETECTION

ANALYTE:

Ribavirin

EXTRACTION:

3 mL H 2SO 4 (pH = 2.5), agitate 30 min

INJECTION VOLUME:

30 µL

MOBILE PHASE:

H 2SO 4, (pH = 2.5), isocratic

FLOW RATE: 1 to 4 L/min VOL-MIN: -MAX:

5 L @ 0.4 mg/m 3 1000 L

SHIPMENT:

routine

SAMPLE STABILITY:

stable in dark at room temperature [1]

TEMPERATURE:

65 °C

BLANKS:

2 to 10 field blanks per set

FLOW RATE:

0.6 mL/min

COLUMN:

30 cm x 7.8 mm, cation exchange resin

DETECTOR:

UV, 210 nm

CALIBRATION:

standard solutions of Ribavirin in H 2SO 4 (pH = 2.5)

RANGE:

2 µg to 2000 µg per sample [1, 2]

ACCURACY RANGE STUDIED:

not studied

BIAS:

unknown

ˆ ): unknown OVERALL PRECISION (S rT

ESTIMATED LOD: 0.7 µg per sample [1, 2]

ACCURACY:

PRECISION (Sr):

unknown

0.057 @ 19 to 112 µg per sample [1, 2]

APPLICABILITY: The working range is 0.04 to 40 mg/m 3 for a 50-L air sample.

INTERFERENCES: None known.

OTHER METHODS: This method is a modification of a bulk assay procedure developed by Eastman Kodak Company [3].

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94