Page:NIOSH Manual of Analytical Methods - 5031.pdf/1
ASPARTAME
C14H18N2O5
MW: 294.34
METHOD: 5031, Issue 2
CAS: 22839-47-0
5031
RTECS: WM3407000
EVALUATION: PARTIAL
OSHA : no standard NIOSH: no standard ACGIH: no standard
Issue 1: 15 August 1990 Issue 2: 15 August 1994
PROPERTIES: solid; MP 248-250 °C
SYNONYMS: L-aspartyl-L-phenylalanine methyl ester; 3-amino-N-(alpha-carboxyphenethyl)-succinamic acid N-methyl ester
SAMPLING SAMPLER:
FILTER (1-µm, 37-mm PTFE)
FLOW RATE:
1 to 3 L/min
VOL-MIN: -MAX:
70 L @ 0.1 mg/m 3 1200 L
SHIPMENT:
routine
SAMPLE STABILITY:
stable at least 30 days at 20 °C [1]
FIELD BLANKS:
2 to 10 field blanks per set
MEDIA BLANKS:
at least 3 per sample set
ACCURACY
MEASUREMENT TECHNIQUE:
HPLC, UV DETECTION
ANALYTE:
Aspartame
EXTRACTION:
2 mL of eluent B.
INJECTION:
25 µL
COLUMN:
C 18 , 10-µm particle size
DETECTOR:
UV, 220 nm
MOBILE PHASE:
60% eluent A/40% eluent B, isocratic
CALIBRATION:
standard solutions of Aspartame in eluent B
RANGE:
7 to 800 µg per sample [1]
RANGE STUDIED:
not studied
ESTIMATED LOD:
2 µg per sample [1]
BIAS:
not determined
PRECISION (Sr):
0.026 @ 4.4 to 435 µg per sample [1]
ˆ ): not determined OVERALL PRECISION (S rT ACCURACY:
not determined
APPLICABILITY: The working range is 0.07 to 8 mg/m 3 for a 100-L air sample. The method has been used to analyze personal and area air samples during a health hazard evaluation at a commercial food packaging plant. [1]
INTERFERENCES: Food additives such as flavoring (artificial flavors), stabilizers (ascorbic acid) and food colors (FD&C yellow) collected along with Aspartame sampling, did not affect the analysis. [1]
OTHER METHODS: An HPLC method for the analysis of Aspartame in bulk has been reported. [2]
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94