Page:NIOSH Manual of Analytical Methods - 5044.pdf/4

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EST RO GE NIC C OM PO UN DS: M ETH OD 5044, Issue 1, dated 1 5 Ma rch 2003 - Page 4 of 6 REFERENCES: [1] [2] [3]

Mathews ES and Neum eister CE [2000]. Backup Data Report for Method 5044, Estrogenic Compounds by HPLC (unpublished), NIOSH, DART. Neum eister CE [1983]. Analytical Report for Sequence 1400. Cincinnati, OH: National Institute of Oc cup ationa l Safe ty and H ealth (u npu blishe d). Sixth Annual Report on Carcinogens [1991]. USDHHS/PHS, National Toxicology Program

METHOD WRITTEN BY:

Elaine S. Mathews and Charles E. Neumeister NIOSH, DART

TABLE 1. PROPERTIES Compound

Formula

M.W.

M.P. (O C)

UV Max (nm)

$-Estradiol

C18 H24 O2

272

173-179

206

Estrone

C18 H22 O2

270

254-256

198

Progesterone

C21 H30 O2

314

127-131

237

$-Estradiol-3-Benzoate

C25 H28 O3

376

191-196

201

NIOSH Manual of Analytical Methods (NMAM), Fourth Edition

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