Page:NIOSH Manual of Analytical Methods - 8004.pdf/1
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| POLYCHLOROBIPHENYLS in serum | 8004 |
| C12H10-xClx (x = 1 to 10) | MW: 188 to 498 | CAS: Varies | RTECS: Varies |
| METHOD: 8004, Issue 2 | EVALUATION: PARTIAL | Issue 1: 15 February 1984 Issue 2: 15 August 1994 |
BIOLOGICAL INDICATOR OF: exposure to PCB.
SYNONYMS: Aroclor; PCB; chlorodiphenyl
BIOLOGICAL SAMPLING
| SPECIMEN: | serum |
| VOLUME: | 10 mL |
| PRESERVATIVE: | none |
| SHIPMENT: | polyethylene shippers @ 10 °C |
| STABILITY: | indefinite if serum is kept frozen |
| CONTROLS: | collect 3 specimens from unexposed populations per study or 1 per 10 unknowns for studies exceeding 30 specimens |
| BULK: | submit a bulk sample of material to which workers were exposed |
MEASUREMENT
| TECHNIQUE: | GAS CHROMATOGRAPHY, ECD |
| INJECTION VOLUME: | 3 µL, Grob type splitless |
| COLUMN: | capillary, 15 m x 0.2-mm ID, WCOT SE-54 |
| CARRIER GAS: | 5% methane in argon, 1mL/min |
| TEMPERATURE-INJECTOR: | 260 °C |
| -COLUMN: | 100 °C for 2 min; 20 °C/min to 260 °C for 4 min |
| -DETECTOR: | 350 °C |
| CALIBRATION: | hexane solutions of Aroclors 1016, 1221, 1232, 1242, 1248, 1254 and 1260 |
| QUALITY CONTROL: | pooled or spiked serum |
| RANGE: | 0.005 to 1.0 µg/mL |
| ESTIMATED LOD: | 0.001 µg/mL |
| RECOVERY: | greater than 80% |
| PRECISION (Sr): | 0.16 |
| ACCURACY: | > ± 30% |
NIOSH Manual of Analytical Methods (NMAM), Fourth Edition, 8/15/94